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That is a major problem because concentration estimates are just that estimates generated by procedures neither designed nor validated to perform any measurement

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[DOI] [PubMed] [Google Scholar] 10.Skrobucha A, Pindlowski P, Krajewska N, Grabowski M, Jonik S

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To test whether the regulation of CYP8B1 by T3 would have any effect on BA composition, we performed UFLC-Triple-time of flight/MS-based metabolite profiling to quantitate the BA levels and found that the percentage of 12-hydroxylated (12-OH) BAs was reduced, while the proportion of non-12-hydroxylated (non-12-OH) BAs was increased in the gallbladder bile, ileum, liver, serum, and urine after five days of T3 treatment in MMI mice (Fig

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