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After the corresponding treatment, cells were fixed with cold methanol for 5 min, blocked with 1% BSA in phosphate-buffered solution (PBS) for 1 h, and then incubated overnight at 4 C with anti-collagen 1 type I antibody (Santa Cruz), anti-collagen IV (20421, Novotec, Lyon, France), collagen 1 type III (sc271249, Santa Cruz), anti-fibronectin (sc8422, Santa Cruz) and LOX (ab174316, Abcam), followed by the corresponding fluorescent secondary antibodies

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The first twin succumbed to rapid deterioration from severe anemia and the second twin required exchange transfusions but, in both cases, there were no Heinz bodies in the blood film or hemoglobin in the urine (52)

Genetically distinct subsets within ANCA-associated vasculitis