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Almost everything we know mechanistically comes from preclinical studies

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The lower specific growth rates can be explained by a combination of metabolic burden on the cell imposed by the overexpression of the introduced genetic constructs, and uncharacterized effects on strain growth behavior commonly encountered as a consequence of using auxotrophic markers

Protein expression was detected using GPX4 antibody and visualized using secondary antibody conjugated with HRP and Pierce ECL Western blotting substrate (Thermo Fisher Scientific) as the substrate of HRP