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Valentic TR

Quantification of GSH using Ellmans assay Cells were harvested and lysed with 0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5 to afford protein free lysate

Figure 3 Overall, individuals in the T2DM group had significantly decreased levels of the reduced form of GSH and increased levels of the oxidative stress marker MDA, compared to the healthy group ( Figures 3AC )

Once oxidised, glutathione penetration is more challenging and also oxidised glutathione has less efficacy as compared to the reduced glutathione

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the first patient with a severe phenotype who was treated with selenium showed a good prognosis (18)