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Cells were labelled with SNAP-Surface Alexa Fluor 546 (New England Biolabs) and SNAP-expressing fluorescent cells sorted by fluorescence-activated cell sorting (FACS) and maintained as above with media supplemented with 0.5 mg/mL G418

The GIP mechanism may explain why some patients respond better to tirzepatide than semaglutide, even at lower nominal doses

10.1126/scitranslmed.aaf3634 36 DongW

Interaction between the non-catalytic cysteine at position 129 and the catalytic cysteine 227 shows the formation of a disulfide bond, which maintains its catalytic activity (91, 92)