The standard dose is 1000 mcg, given as an intramuscular or subcutaneous injection
This is because though cobalamin will take a couple of weeks to reach appropriate levels in the body, especially if deficient, it can last in the body for a long time once it reaches appropriate concentrations
B1 has also increased my energy & may have lowered my arthritis pain too
The key word in all of this is deficient, because that is where the benefit lives
Here is a quick storage guide Before reconstitution (powder) Storage : Refrigerate at 2-8C (36-46F) Shelf life : 2-3 years when properly stored Freezer : Can freeze for longer storage (3-5 years) Room temperature : Survives short periods (days to weeks) but refrigeration preferred After reconstitution (liquid) Storage : Refrigerate immediately at 2-8C Shelf life : 28 days with bacteriostatic water Freezer : Not recommended (can damage peptide structure) Room temperature : Begins degrading within hours, use within days max Key rules Always refrigerate reconstituted peptides Use bacteriostatic water (not sterile water) Mark reconstitution date on vial Protect from light (store in box) Use within 28 days after reconstitution Never shake vials vigorously Understanding peptide stability Why peptides degrade Chemical breakdown : Peptide bonds can break down over time, especially in liquid form

Vitamin B12 is essential in the formation of red blood cells and helps in the absorption of iron in the body