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How GLP-1 Is Made (Proglucagon Pathway) The production of GLP-1 begins with a larger precursor molecule

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The above Syn constructs were transformed into BL21(DE3) cells induced with 0.5 mM isopropyl -D-1-thiogalactopyranoside (IPTG), followed by post-induction incubation at 37C for 5 h

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Using bacteriostatic water helps, as it contains 0.9% benzyl alcohol to inhibit growth, but it isn't a substitute for a cold, dark environment