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The cells were washed with 0.1 M potassium phosphate buffer, pH 7.5, resuspended in ice-cold 8 mM HCl, 1.3% (w/v) 5-sulfosalicylic acid solution and disintegrated with glass beads (particle size 425600 m) in a bead mill for 3*30 s at maximum speed

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Citrulline is generally well-tolerated with fewer gastrointestinal side effects than arginine

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Lower-dose oral semaglutide (714 mg) is already approved under the brand name Rybelsus for type 2 diabetes

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