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If there is presence of enzymatic activity and/or spontaneous oxidation in storage, we would expect to observe lower levels of total GSH and free GSH, and elevated levels of GSSG, in the EDTA-treated sample stored at 4 o C compared to the non-deproteinized EDTA-treated plasma stored at 80 o C

Also, NOX2 was upregulated in embryonic fibroblasts obtained from C9orf72 knockout mice, suggesting that cellular redox conditions are dysregulated by depletion of C9orf72 (Liu Y