Following incubation for 1 h at 37 C, plates were washed with PBST and 3,3',5,5'-Tetramethylbenzidine (TMB Sera care TMB-51200047) was added to all wells and incubated for 5 min at room temperature (RT)
In such a way, it contributes to the proper functioning of the nervous system
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For best practices in a research setting, you should use one syringe for reconstitution and a fresh, sterile syringe (typically an insulin syringe) for drawing each precise dose for your experiment
