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Anti-mouse or anti-rabbit secondary antibodies (Cell Signaling Technology) HRP-conjugated at 1:5000 dilution were incubated with the membranes for 1 h at room temperature, and visualized using a chemiluminescence system
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While effective for symptom control, NSAIDs carry risks of gastrointestinal ulceration and bleeding, cardiovascular events, renal impairment, and impaired bone and tendon healing
Jennings JH, Rizzi G, Stamatakis AM, Ung RL, Stuber GD