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However, when generating paired libraries using these standard approaches, we observe three points of inefficiency(1) limited or skewed diversities of captured variable regions from lysed B cells, (2) the stochastic movement of mRNA transcripts during the transfer of capture beads between emulsions and (3) droplet merging events during in-drop OE RT-PCRthat affect both the preservation of cognate pairing and sequence diversity within the repertoire