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These changes occurred through direct effects on adipocyte biology rather than appetite suppression, as research models maintained normal feeding behavior while exhibiting significant reductions in individual adipocyte size

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Using quantified PCR (qPCR), we first confirmed the TANGO2 expression in TANG016+Ad-WT at transcripts level, while no transcription of full-length TANGO2 was detected in TAN016+Ad-GFP (Figure 2C)