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After confirming that the concentration of NADPH in our lysates was within the linear detection range of the assay (Fig 3A), we treated LPS-stimulated (6 h) RAW264.7 Ms with 2-DG and diamide and quantified NADPH kinetics by obtaining lysates at 5-minute intervals for a total of 25 minutes (Fig 3B)

Cysteine Contribution for Gluconeogenesis and Phosphate Pentose Pathway (PPP) Gluconeogenesis or the synthesis of glucose from non-glucidic compounds, such as glycerol, lactate, pyruvate, acetyl-CoA, or glucogenic AA, only recently started to be explored in cancer