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J Biol Chem (2009) 284(16):10890900.10.1074/jbc.M807994200 47 Pacheco-PantojaELDillonJPWilsonPJFraserWDGallagherJA

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Reductase enzymatic activity The reductase activities of the WT and mutant (K8A, C20A and K47A) proteins were measured spectrophotometrically by recording the increase in absorbance at 260 nm using a UV-VIS spectrophotometer (UV-1800, Shimadzu, Kyoto, Japan) as described previously 37