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EC50 determination is reliable Scenario B: 85% pure IGF-1 LR3 stock (1 mg/mL) At a nominal concentration of 100 ng/mL in assay, actual IGF-1 LR3 = ~85 ng/mL Remaining ~15 ng/mL consists of potentially inhibitory, partially active, or bioactive contaminant species Dose-response curve is shifted

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UV radiation comprises three wavelengths: UVA, UVB, and UVC