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GRADE guidelines: 5

The samples were centrifuged at 1000 rpm for 3 min using a KUBOTA 3520 (KUBOTA, Tokyo, Japan), and absorbance was measured at 595 nm using a GeneQuant 1300 spectrophotometer (Biochrome, Cambridge, UK)

Subsequently, basal NPH insulin was discontinued due to good glycemic control

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444 The expression of nerve growth factor-inducible protein A (NGFI-A), an enzyme that bindss exon 1 F of GR, is reduced in the hippocampus of patients with MDD, which may contribute to low methylation levels in the brain