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Thereafter, the mixtures were centrifugated at 10,000 rpm for 15 minutes duration using an Eppendorf centrifuge 5810/5810 R series

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In isolated human islets, we confirmed that hGIP stimulated glucagon secretion, GLP-1 decreased glucagon secretion and the combination of the two peptides offset each other to produce a rate of glucagon secretion that matched unstimulated levels (Fig