10.2527/jas.2007-0468 212 ProvenzaF
Dual luciferase reporter assay For the luciferase assay, 293 T cells were cultured in 24-well plates at 70 to 80% confluency and co-transfected with the plasmids encoding Ppara -wild, Ppara -mut, Pparg -wild, or Ppard- wild, along with the PPRE plasmid and a control vector (Renilla luciferase), using Lipofectamine 3000 Reagent (Invitrogen, L3000150) according to the manufacturers protocols
The results showed significant improvement, versus the placebo, in hydration, pigmentation, wrinkles, and texture
For the trial-by-trial numbers, see what each GLP-1 medication actually delivers
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