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Caspase 3 activity assay Fifty milligrams of frozen remnants livers were lysed in caspase 3 activity buffer (20 mM PIPES, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% CHAPS, 10% sucrose, pH 7.2)

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However, there are important caveats: It is not FDA-approved for any medical treatment, so its use is considered off-label. BPC-157 is reported safe in recommended dosages

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