In a clinical study conducted by Stracquadanio et al., continuous administration of myo-inositol, gymnemic acid, and L-methylfolate for 6 months demonstrated substantial beneficial effects in PCOS patients, including improvement in menstrual cycle regularity and metabolic parameters, reduction in BMI and total testosterone, and increased insulin sensitivity (229)
The standards or samples were then transferred to a 96-well plate in duplicate with 50 L per well and 50 L chlorinating reagent (197 mM Na 2 HPO 4 and 0.6% (v/v) NaOCl) was added
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