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Moreover, incubation of the [C-RML][ S 1 ] melt for 1 h at 150 C followed by cooling to 30 C showed almost complete retention of the enzymatic activity when pNPB was added (Supplementary Fig

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The relatively simple measurement, inexpensive instrumentation, and robustness and potential for high-throughput assay make AuNP-based colorimetric assays excellent candidates for in vitro measurements of enzyme activity