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Induction of prostatic inflammation by the activation of the AR, GPER-Gai-EGFR/PI3K/ERK, or NF-B pathway as the nuclear transcriptional endpoint

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Upon terminating digestion, the cells were collected in the corresponding centrifuge tubes by a centrifugation (1000 rpm, 5 min)

These findings positioned DES(1-3) IGF-1 as a standard pharmacological tool for IGF1R-dependent resistance studies in oncology research