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Sections were dewaxed and stained with a rabbit polyclonal anti-FITC (711900, Thermo Fisher Scientific, 1/100) plus secondary Alexa Fluor 488 antibody (Life Technologies, 1/200), and guinea pig anti-human insulin (A0564, Dako, 1/1000) plus secondary Alexa Fluor 546 antibody (Life Technologies, 1/1000) prior to confocal imaging as above

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Results Patients started semaglutide 64.7 47.6 months (mean SD) after BS

For PMDD night sweats, that can mean less tossing, fewer wake ups, and less of that sticky, overheated feeling