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The enriched IGF-1/LR3 eluate is dried in a speed vac to remove TFA, and then reconstituted for subsequent reduction, alkylation, and tryptic digestion

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Such models are used to evaluate signaling interactions, biomarker expression, and structural organization under controlled laboratory conditions, with emphasis on reproducibility and mechanistic interpretation rather than applied outcomes

Co-eluting, high abundant interferences in TOFMS survey scans resulted in missed MS/MS acquisition of target peptides

Drosophila stocks and generation of UAS-HA-V5-LaccID flies Flies were maintained on standard cornmeal medium on a 12-h lightdark cycle at 25 C