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55 Morote-GarciaJ

Roche NGS-based genotyping A NGS prototype provided by Roche Diagnostics (Roche-NGS) was used on an Illumina platform with the purpose of analyzing the NS5B region of HCV genomes as previously described 12

RNA-Seq libraries were prepared from 200 ng of total RNA with the Illumina TruSeq Stranded mRNA reagents (Illumina) using a unique dual indexing strategy and following the official protocol automated on a Sciclone liquid handling robot (PerkinElmer)

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