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For the 2,4,6-trinitrobenzene sulfonic acid (TNBS) model, mice were shaved on the back just below the neck and painted with 1% TNBS (Meilunbio, MB5523) mixed in an acetone/olive oil solution for presensitization (150 L per mouse)

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The timeline is gradual: better sleep and energy at 4 weeks, less bloating and improved recovery at 8 weeks, measurable body composition changes by 12 weeks

SGLT2 inhibitors (empagliflozin, dapagliflozin, canagliflozin) increase urinary glucose excretion, creating a glucose-rich environment in the genitourinary tract that promotes fungal overgrowth