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12 week-old mice) ( Paracellular transport across the epithelial barrier is regulated by tight junction proteins, which form cell-to-cell complexes and depend on calcium for assembly ( Cldn3, Ocln, Tjp1 (ZO-1), and Jam1 in the small intestine is lower in newborns (1 day and 2-week-old mice), while the expression of Cldn2 is higher compared to adults (12-week-old mice) ( Intestinal alkaline phosphates is a brush border enzyme expressed and secreted by enterocytes ( in vit ro ( in vivo ( in vivo studies have shown that serum alkaline phosphatase levels are higher in young children (29 days to 1 year, 50 th percentile: males = 218.19, females = 210.62 U/L) compared to older children (17 to 18 years, 50 th percentile: males = 104.74, females = 75.50 U/L) ( Permeability is also influenced by the mucus layer, composed of high-molecular-weight glycoproteins secreted by goblet cells ( in vivo data show that at birth colonic mucus in humans, pigs and mice predominantly contains acidic mucins over neutral mucins ( Differences in paracellular permeability, tight junctions, and mucus properties between infants and adults question the reliability of adult gut models for infant research

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Data collection and clinical laboratory testing Demographic characteristics, including age, sex, body mass index (BMI), and use of lipid-lowering therapy, were recorded for each participant

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