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secondly, it reduces oxidative stress and lipotoxicity by scavenging free radicals and removing excess palmitate from the ER, and finally it promotes oocyte growth and maturation by decreasing the rate of apoptosis

The tissue was then digested for 15 min in a 37 C shaking water bath incubator using 5 ml of 100 U/ml collagenase type I (Sigma) in Basal Medium Eagle (Thermo Fisher Scientific) and supplemented with 1% BSA, 26.4 mM N a HCO 3 , and 10 mM HEPES (pH 6.9)

Arits AHMM, Mosterd K, Essers BAB, Spoorenberg E, Sommer A, De Rooij MJM, et al

Marshall, S., Garvey, W

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doi: 10.1007/BF03345187 51 HernndezJJCGarcaJMMGarca DiezLC