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Mean age at lumbar puncture was not significantly different (63.80 vs 67.89, p = 0.17, ICH+ vs ICH-)

For optogenetic experiments, AgRP-Cre mice were crossed with B6.Cg- Gt(ROSA)26Sor tm32(CAG-COP4*H124R/EYFP)Hze /J mice (ROSA26-loxStoplox-ChR2-eYFP, #024109, Jackson Labs), to generate AgRP:ChR2 animals

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