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Low allopurinol doses are sufficient to optimize azathioprine therapy in inflammatory bowel disease patients with inadequate thiopurine metabolite concentrations

Paw withdrawal threshold was calculated from the average of two trials, using SemmesWeinstein von Frey monofilaments with bending forces of 0.008, 0.02, 0.04, 0.07, 0.16, 0.4, 0.6, and 1.0 g) applied in ascending order to the plantar surface

Cells were detached with EDTA, resuspended in HBSS, and furimazine (Promega) was added at a 1:50 dilution from the manufacturers pre-prepared stock

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The products were obtained by silica gel column chromatography

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