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These groups as follows: 1) vehicle control (VC) group receiving equal amount of 1% DMSO in physiological saline and DMEM (Zhao et al., 2023) initially, followed by physiological saline (0.3 mL/rat) after 30 min, 2) control negative (CN) or PTZ treated group receiving equal amount of 1% DMSO in physiological saline and DMEM initially, followed by the subthreshold dose of PTZ (35 mg/kg) after 30 min, 3) control positive (CP) group receiving PB (30 mg/kg) 30 min before the PTZ injection, (4, 5 and 6) groups receiving different doses of glycitin (5, 10, and 20 mg/kg) respectively, followed by PTZ after 30 min