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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

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While early-stage, these findings support the use of GHK-Cu in experimental pain models that do not rely on classical opioid or NSAID pathways

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Cholinesterase inhibitors (donepezil, rivastigmine) - Theoretical complementary mechanisms cholinesterase inhibitors increase acetylcholine while dihexa builds new synapses