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The N-terminal segment (amino acids 1130, Pelo-N), middle segment (amino acids 136268, Pelo-M), and C-terminal segment (amino acids 271370, Pelo-C) of Pelo was inserted into the prey vector pGADT7 to generate pGADT7-Pelo-N, pGADT7-Pelo-M, and pGADT7-Pelo-C, respectively

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