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At the end of incubation, 20 L of MTS reagent was added to each well and incubated for another two hours, then analyzed with a microplate reader using a test wavelength of 490 nm within 10 min

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doi: 10.1080/1028415X.2022.2146843 Summary Keywords intermittent fasting, immune system, immunomodulatory effect, metabolic syndrome, obesity Citation He Z, Xu H, Li C, Yang H and Mao Y (2023) Intermittent fasting and immunomodulatory effects: A systematic review