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At the end point, cells were collected by trypsinization (the culture medium and PBS washes were collected and re-added to each sample) and washed once with assay buffer (10 mM HEPES, 140 mM NaCl and 3.3 mM CaCl 2 , pH 7.4)

Evaluation of Launaea procumbens use in renal disorders: a rat model

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For generating calibration curve of HT1080 cells, exact same conditions were used as described above