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After 24 h medium was discarded as recommended in [9] and 110 l medium without CuCl 2 or CuSO 4 containing 10 l CCK-8 Solution was added

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IV therapy involves administering nutrients directly into your bloodstream via an intravenous catheter

10.1007/s11357-013-9514-2 28 LangmeadB.SalzbergS

Curr Opin Cell Biol 15:247254 Smythies J (1999) The neurotoxicity of glutamate, dopamine, iron and reactive oxygen species: functional interrelationships in health and disease: a reviewdiscussion

If there is clinical improvement and if a reticulocyte response is observed, the same amount may be given on alternate days for seven doses, then every three to four days for another two to three weeks