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For method 2 sample preparation, the initial sample preparation was the same as described for method 1, except that NEM was added to the extraction solvent (50 L of 50 mM NEM in 80% methanol, 20% 10 mM ammonium formate, pH 7.0 and 50 L of 100% ACN)

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Hypoglycemia represents a more serious potential complication, particularly for patients with diabetes

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