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Using affinity-purification mass spectrometry, Gordon et al

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Furthermore, after pull-down with the anti-ERK5 antibody in Huh-7 cells, we observed smeared bands of ubiquitination in the presence of bortezomib (BTZ) and RNK06138, which implied the subsequent proteasomal degradation of ERK5 after ternary complex formation with E3 ubiquitin ligases recruitment to ERK5 (Fig

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