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Immunofluorescence assay Vero cells were grown in micro-tissue chamber slides at a concentration of 4104 cells/well for 24 hrs in 5% CO2 at 37C

The role of macrophage iron overload and ferroptosis in atherosclerosis

It has been reported that skeletal muscle is an important deposit of MeHg [188] and the activated antioxidant defense system of cells provides a compensatory mechanism for HgCl 2 induced oxidative stress

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Ultrapure water was from a Millipore Synergy UV system (Billerica, MA)