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Cell transfection Cells were transfected with pcDNA 3.1 overexpression vectors (pcDNA 3.1-YTHDF1 and pcDNA 3.1-SLC7A5, with the empty pcDNA 3.1 vector as a negative control) and siRNAs (Invitrogen) targeting YTHDF1 and SLC7A5 (si-YTHDF1, si-SLC7A5, with non-targeting siRNA (si-NC) as a negative control)

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These in-organ changes in energy metabolism are also reflected in the plasma metabolome after ischemic AKI, which is characterized by the depletion of numerous amino acids and energy substrates 6