Non-specific bindings were blocked by incubation with 5% non-fat dry milk in Tris-buffered saline Tween (TBST) for 2 h
Berberine Berberine is a natural compound found in various plants, like barberry, goldenseal, and Oregon grape
GHK-Cu Versus Thymosin Beta-4 Thymosin Beta-4 (TB-500) promotes cellular migration and angiogenesis, essential processes for wound healing
Further, the SOE PCR products were cloned into FastDigest BshTI/FastDigest BglII (Thermo Fisher) linearized pEGFP-C2 vector using ClonExpress II One Step Cloning Kit (C112, Vazyme) to obtain the six sorting vectors, pEGFP-GST-GPI DAF , pEGFP-GST-GPI BY55 , pEGFP-GST-GPI CEAM7 , pEGFP-GST-TMD ITB3 , pEGFP-GST-TMD ITA5, and pEGFP-GST-TMD ITAV
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