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Unless indicated otherwise, cystine-free medium was prepared by supplementing cystine-, methionine- and glutamine-free DMEM (Gibco, CA, USA) with 100 M methionine, 4 mM glutamine, 1 mM sodium pyruvate, 0.2% bovine serum albumin (BSA), 100 U/ml penicillin and 100 g/ml streptomycin

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For example, if you added 4 mL instead of 3 mL to a 15 mg vial, your new concentration is 15 mg / 4 mL = 3.75 mg/mL

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Some users abandon protocols prematurely and miss out on benefits that would have developed with continued use