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While not strictly necessary, allowing both the peptide and bacteriostatic water vials to sit at room temperature for a few minutes can make handling easier

IGF1R autophosphorylation also recruits Shc, which leads to Grb2/SOS/Ras activation and downstream ERK1/2 phosphorylation

Glutathione (GSH) is an intracellular tripeptide antioxidant that significantly contributes to maintaining optimal redox balance, which may impact bacterial survival during antibiotic treatment

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however, they may not be precise enough in all cases