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Splenocytes were washed with warm RPMI 1640, stained for surface markers detection at 37 C for 30 min in RPMI 1640, and washed with PBS

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In three studies, several patients did not complete the study and had a high risk of bias in the incomplete outcome data domain (Reference Oner and Muderris11,Reference Kose and Naziroglu26,Reference Fulghesu, Ciampelli and Muzj29)

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