Stamatovic SM, Keep RF, Andjelkovic AV
After blocking with TBST containing 5% nonfat milk for 1 h, the membranes were incubated with GPX4 (rabbit, 22 kDa, ab125066, 1:5,000, Abcam), 4-hydroxynonenal (4-HNE) (rabbit, 1776 kDa, ab46545, 1:2,500, Abcam), acyl-CoA synthetase long-chain family member 4 (ACSL4) (mouse, 75 kDa, sc-365230, 1:1,000, Santa Cruz), solute carrier family 7 member 11 (SLC7A11) (rabbit, 55 kDa, ab175186, 1:5,000, Abcam), Anti-5 Lipoxygenase (5-LOX) (rabbit, 78 kDa, ab169755, 1:1,000, Abcam) and -actin (mouse, 43 kDa, A5441, 1:5,000, Sigma) overnight at 4C
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Other mechanisms on which Metformin play an action are: mammalian target of rapamycin (mTOR, crucial to tumor cell metabolism), adenosine mono-phosphate-activated protein kinase (AMPK), mitochondrial glycerophosphate dehydrogenase (mGPDH), and the nuclear factor kB (NF-kB) (Yoshida et al., 2020)
Rather than treating individual risk factors in isolation, this triple agonist peptide addresses the underlying metabolic dysfunction that drives cardiovascular disease
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