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Excessive copper binds selectively to lipoylated proteins involved in the tricarboxylic acid (TCA) cycle, leading to proteotoxic stress and cell death by increasing ROS [170, 171]

Data represent mean SD

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Additionally, we extended our focus to RIP1 and RIP3, which are key inducers of necroptosis in the early phase of APAP toxicity, manifesting effects within 2 h of administration (Liu et al