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The cultures were then centrifuged at 6,000 rpm for 10 min at 4C, after which the bacterial pellets were washed twice with PBS

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Psychological and quality of life changes in patients using GLP-1 analogues

The effect of vitamin D to up-regulate PD-1/PDL-1 may restore the immune tolerance and contribute to prevent the autoimmune response in AA (Figure 3B)