Growth of methionine synthesis mutants in laboratory cultures The L-methionine synthesis mutants were first grown in 7H9-Tween-ADC liquid medium, both with and without B12 (AdoCbl, 10 g/mL), to assess their growth in the presence and absence of the vitamin and thus validate their phenotypes
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Conflict of interest The authors have no conflict of interest to declare.
Individuals with B12 Deficiency B12 deficiency symptoms include fatigue, weakness, numbness or tingling in the extremities, tongue soreness, depression, and even memory issues