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*Correspondence: Piero Sestili, [email protected] This article was submitted to Respiratory Pharmacology, a section of the journal Frontiers in Pharmacology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

Multiple needle punctures into a non-preserved vial raise contamination risk, so single-use or short-window protocols are safer